NBP247862PE

Nucleolin Antibody (364-5 + NCL/902), PE, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NBP247862PE-Each-of-1 In Stock ₹ 55,271.76

NBP247862PE - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

Nucleolin

Classification

Monoclonal

Conjugate

PE

Formulation

PBS with 0.05% Sodium Azide

Gene Symbols

NCL

Immunogen

Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity

0.1 mL

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage

Store at 4C in the dark.

Isotype

IgG1 κ

Applications

Flow Cytometry

Clone

364-5 + NCL/902

Dilution

Flow Cytometry

Gene Alias

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species

Mouse

Purification Method

Protein A or G purified

Research Discipline

Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez)

4691

Target Species

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form

Purified

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Description

  • Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
  • It is validated for Flow Cytometry.

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Img

Novus Biologicals

NBP247862PE

--


Antigen:
Nucleolin

Classification:
Monoclonal

Conjugate:
PE

Formulation:
PBS with 0.05% Sodium Azide

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
364-5 + NCL/902

Dilution:
Flow Cytometry

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Img

Novus Biologicals

NBP247862UV

--


Antigen:
Nucleolin

Classification:
Monoclonal

Conjugate:
DyLight 350

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
364-5 + NCL/902

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
4691

Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Form:
Purified

Img

Fischer Scientific

NBP247864A

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Antigen:
VCAM-1/CD106

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
VCAM1

Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunofluorescence, CyTOF

Clone:
B-K9

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers

Gene ID (Entrez):
7412

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247864B

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Antigen:
VCAM-1/CD106

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
VCAM1

Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)

Quantity:
0.2 mg

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunofluorescence, CyTOF

Clone:
B-K9

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers

Gene ID (Entrez):
7412

Target Species:
Human

Form:
Purified