NBP247938B

Elastase Antibody (CELA3B/1218) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

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Antigen

Elastase

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias

CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Host Species

Mouse

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG κ

Applications

Flow Cytometry, ELISA, Immunofluorescence, CyTOF

Clone

CELA3B/1218

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

CELA3B

Immunogen

Recombinant human CELA3B protein

Quantity

0.2 mg

Research Discipline

Cancer, Neuroscience

Gene ID (Entrez)

23436

Target Species

Human

Form

Purified

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Description

  • Elastase Monoclonal specifically detects Elastase in Human samples
  • It is validated for Western Blot, ELISA, CyTOF-ready.

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Fischer Scientific

NBP247938B

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Antigen:
Elastase

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG κ

Applications:
Flow Cytometry, ELISA, Immunofluorescence, CyTOF

Clone:
CELA3B/1218

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
CELA3B

Immunogen:
Recombinant human CELA3B protein

Quantity:
0.2 mg

Research Discipline:
Cancer, Neuroscience

Gene ID (Entrez):
23436

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247939A

--


Antigen:
Cytokeratin 7

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
KRT7/760

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
KRT7

Immunogen:
Recombinant full-length human KRT7 protein

Quantity:
0.1 mg

Research Discipline:
Cytoskeleton Markers

Gene ID (Entrez):
3855

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247939B

--


Antigen:
Cytokeratin 7

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
KRT7/760

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
KRT7

Immunogen:
Recombinant full-length human KRT7 protein

Quantity:
0.2 mg

Research Discipline:
Cytoskeleton Markers

Gene ID (Entrez):
3855

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP247940A

--


Antigen:
Cytokeratin 7

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Simple Western : 1.0 ug/ml, Flow Cytometry : 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin : 1:100-1:200, Immunofluorescence : 1:50 - 1:100, CyTOF-ready

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
OV-TL12/30

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
KRT7

Immunogen:
OTN 11, ovarian carcinoma cell line

Quantity:
0.1 mg

Research Discipline:
Cytoskeleton Markers

Gene ID (Entrez):
3855

Target Species:
Human

Form:
Purified