NBP247964B

CD55/DAF Antibody (143-30) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

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Antigen

CD55/DAF

Classification

Monoclonal

Concentration

1.0 mg/mL

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias

CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC

Host Species

Mouse

Molecular Weight of Antigen

70 kDa

Quantity

0.2 mg

Research Discipline

Immunology

Gene ID (Entrez)

1604

Target Species

Human

Form

Purified

Applications

Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF

Clone

143-30

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

CD55

Immunogen

PHA stimulated human PBL

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG1 κ

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Description

  • CD55/DAF Monoclonal specifically detects CD55/DAF in Human samples
  • It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Flow (Cell Surface), Immunofluorescence, CyTOF-ready.

Compare Similar Items

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Fischer Scientific

NBP247964B

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Antigen:
CD55/DAF

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Gene Alias:
CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC

Host Species:
Mouse

Molecular Weight of Antigen:
70 kDa

Quantity:
0.2 mg

Research Discipline:
Immunology

Gene ID (Entrez):
1604

Target Species:
Human

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence, CyTOF

Clone:
143-30

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
CD55

Immunogen:
PHA stimulated human PBL

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

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NBP247965A

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Antigen:
NGF R/TNFRSF16/p75NTR

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Gene Alias:
CD271, CD271 antigen, Gp80-LNGFR, member 16, nerve growth factor receptor, NGF receptor, p75 ICD, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), tumor necrosis factor receptor superfamily member 16

Host Species:
Mouse

Molecular Weight of Antigen:
75 kDa

Quantity:
0.1 mg

Research Discipline:
Apoptosis, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Gene ID (Entrez):
4804

Target Species:
Human, Baboon, Feline, Ferret, Monkey, Rabbit, Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
NGFR5

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NGFR

Immunogen:
NGFR from A875 melanoma cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Img

Fischer Scientific

NBP247965B

--


Antigen:
NGF R/TNFRSF16/p75NTR

Classification:
Monoclonal

Concentration:
1.0 mg/mL

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

Gene Alias:
CD271, CD271 antigen, Gp80-LNGFR, member 16, nerve growth factor receptor, NGF receptor, p75 ICD, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), tumor necrosis factor receptor superfamily member 16

Host Species:
Mouse

Molecular Weight of Antigen:
75 kDa

Quantity:
0.2 mg

Research Discipline:
Apoptosis, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Gene ID (Entrez):
4804

Target Species:
Human, Baboon, Feline, Ferret, Monkey, Rabbit, Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Clone:
NGFR5

Conjugate:
Unconjugated

Formulation:
PBS with No Preservative

Gene Symbols:
NGFR

Immunogen:
NGFR from A875 melanoma cells

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype:
IgG1 κ

Img

Novus Biologicals

NBP247967C

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Antigen:
NGFR/TNFRSF16/p75NTR

Classification:
Monoclonal

Concentration:
__

Dilution:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
CD271, CD271 antigen, Gp80-LNGFR, member 16, nerve growth factor receptor, NGF receptor, p75 ICD, TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16), tumor necrosis factor receptor superfamily member 16

Host Species:
Mouse

Molecular Weight of Antigen:
__

Quantity:
0.1 mL

Research Discipline:
Apoptosis, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Gene ID (Entrez):
4804

Target Species:
Human, Primate, Mouse (Negative), Rat (Negative)

Form:
Purified

Applications:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence

Clone:
NGFR5 + NTR/912

Conjugate:
DyLight 650

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
NGFR

Immunogen:
NGFR from A875 melanoma cells (NGFR5); Recombinant human p75 NGFR protein (NTR/912)

Purification Method:
Protein A or G purified

Regulatory Status:
__

Primary or Secondary:
Primary

Test Specificity:
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1 κ