Classification:
Monoclonal
Formulation:
PBS with 0.02% Proclin 300. with 0.02% Proclin 300
Purification Method:
Protein A or G purified
Test Specificity:
Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Applications:
Flow Cytometry, ELISA, Immunohistochemistry, CyTOF
Clone:
4D5-8 (trastuzumab)
Dilution:
Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, CyTOF-ready
Gene Alias:
CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Immunogen:
A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
Primary or Secondary:
Primary