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NBP77836549

Smad3, p Ser425, p Ser423 Antibody, Janelia Fluor™ 549, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP77836549-Each-of-1 In Stock ₹ 67,763.52

NBP77836549 - Each of 1

₹ 67,763.52

In Stock

Quantity

1

Base Price: ₹ 67,763.52

GST (18%): ₹ 12,197.434

Total Price: ₹ 79,960.954

Antigen

Smad3

Classification

Polyclonal

Dilution

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Gene Alias

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Host Species

Rabbit

Purification Method

Affinity Purified

Research Discipline

Signal Transduction, Wnt Signaling Pathway

Gene ID (Entrez)

4088

Target Species

Human, Mouse, Rat, Porcine, Xenopus species, Zebrafish

Isotype

IgG

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Conjugate

Janelia Fluor 549

Formulation

50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols

SMAD3

Immunogen

Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)

Quantity

0.1 mL

Primary or Secondary

Primary

Test Specificity

This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Content And Storage

Store at 4°C in the dark.

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Description

  • Smad3 Polyclonal antibody specifically detects Smad3 in Human, Mouse samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Compare Similar Items

Show Difference

Img

Novus Biologicals

NBP77836549

--

Antigen:
Smad3
Classification:
Polyclonal
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Host Species:
Rabbit
Purification Method:
Affinity Purified
Research Discipline:
Signal Transduction, Wnt Signaling Pathway
Gene ID (Entrez):
4088
Target Species:
Human, Mouse, Rat, Porcine, Xenopus species, Zebrafish
Isotype:
IgG
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Janelia Fluor 549
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Gene Symbols:
SMAD3
Immunogen:
Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NBP77836646

--

Antigen:
Smad3
Classification:
Polyclonal
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Host Species:
Rabbit
Purification Method:
Affinity Purified
Research Discipline:
Signal Transduction, Wnt Signaling Pathway
Gene ID (Entrez):
4088
Target Species:
Human, Mouse, Rat, Porcine, Xenopus species, Zebrafish
Isotype:
IgG
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Janelia Fluor 646
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Gene Symbols:
SMAD3
Immunogen:
Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NBP79124549

--

Antigen:
LambdaLightChain
Classification:
Monoclonal
Dilution:
Flow Cytometry
Gene Alias:
IGLC, immunoglobulin lambda constant group
Host Species:
Mouse
Purification Method:
Protein A purified
Research Discipline:
Adaptive Immunity, Immunology
Gene ID (Entrez):
3536
Target Species:
Human
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Conjugate:
Janelia Fluor 549
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Gene Symbols:
IGLC1
Immunogen:
Human IgA1 lambda myeloma protein
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.
Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NBP79124646

--

Antigen:
LambdaLightChain
Classification:
Monoclonal
Dilution:
Flow Cytometry
Gene Alias:
IGLC, immunoglobulin lambda constant group
Host Species:
Mouse
Purification Method:
Protein A purified
Research Discipline:
Adaptive Immunity, Immunology
Gene ID (Entrez):
3536
Target Species:
Human
Isotype:
IgG1 κ
Applications:
Flow Cytometry
Conjugate:
Janelia Fluor 646
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Gene Symbols:
IGLC1
Immunogen:
Human IgA1 lambda myeloma protein
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.
Content And Storage:
Store at 4°C in the dark.