NP233144A70

CD45RA Antibody (158-4D3), Alexa Fluor™ 750, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NP233144A70-Each-of-1 In Stock ₹ 55,271.76

NP233144A70 - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

CD45RA

Classification

Monoclonal

Conjugate

Alexa Fluor 750

Formulation

50 mM sodium borate with 0.05% sodium azide

Gene Symbols

PTPRC

Immunogen

Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.

Quantity

0.1 mL

Gene ID (Entrez)

5788

Target Species

Human, Primate

Isotype

IgG2a κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone

158-4D3

Dilution

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Host Species

Mouse

Purification Method

Protein A or G purified

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Content And Storage

Store at 4°C in the dark.

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Description

  • CD45RA Monoclonal specifically detects CD45RA in Human samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Compare Similar Items

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Img

Novus Biologicals

NP233144A70

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Antigen:
CD45RA

Classification:
Monoclonal

Conjugate:
Alexa Fluor 750

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
PTPRC

Immunogen:
Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.

Quantity:
0.1 mL

Gene ID (Entrez):
5788

Target Species:
Human, Primate

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
158-4D3

Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Host Species:
Mouse

Purification Method:
Protein A or G purified

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NP233146A30

--


Antigen:
Insulin

Classification:
Monoclonal

Conjugate:
Alexa Fluor 350

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
INS

Immunogen:
Purified pig insulin, conjugated to KLH (Uniprot: P01308)

Quantity:
0.1 mL

Gene ID (Entrez):
3630.0

Target Species:
Human, Mouse

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
2D11-H5 (same as INS05)

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin

Host Species:
Mouse

Purification Method:
Protein A or G purified

Primary or Secondary:
Primary

Test Specificity:
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NP233146A32

--


Antigen:
Insulin

Classification:
Monoclonal

Conjugate:
Alexa Fluor 532

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
INS

Immunogen:
Purified pig insulin, conjugated to KLH (Uniprot: P01308)

Quantity:
0.1 mL

Gene ID (Entrez):
3630

Target Species:
Human, Mouse

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
2D11-H5 (same as INS05)

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin

Host Species:
Mouse

Purification Method:
__

Primary or Secondary:
Primary

Test Specificity:
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Content And Storage:
Store at 4°C in the dark.

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Novus Biologicals

NP233148A30

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Antigen:
Melan-A/MART-1

Classification:
Monoclonal

Conjugate:
Alexa Fluor 350

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
MLANA

Immunogen:
Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)

Quantity:
0.1 mL

Gene ID (Entrez):
2315

Target Species:
Human, Mouse, Rat

Isotype:
IgG2b λ

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
M2-7C10

Dilution:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A

Host Species:
Mouse

Purification Method:
Protein A or G purified

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Content And Storage:
Store at 4°C in the dark.