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NP234718A94

p27/Kip1 Antibody (SX53G8), Alexa Fluor™ 594, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NP234718A94-Each-of-1 In Stock ₹ 55,271.76

NP234718A94 - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

p27/Kip1

Classification

Monoclonal

Conjugate

Alexa Fluor 594

Formulation

50 mM sodium borate with 0.05% sodium azide

Gene Symbols

CDKN1B

Immunogen

Purified GST-p27 fusion protein of human origin (Uniprot: P46527)

Quantity

0.1 mL

Primary or Secondary

Primary

Test Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Content And Storage

Store at 4°C in the dark.

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone

SX53G8

Dilution

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias

CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1

Host Species

Mouse

Purification Method

Protein A or G purified

Research Discipline

Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors

Gene ID (Entrez)

1027.0

Target Species

Human, Mouse, Rat, Primate

Isotype

IgG1 κ

Description

  • Description p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Novus Biologicals

NP234718A94

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Antigen:
p27/Kip1
Classification:
Monoclonal
Conjugate:
Alexa Fluor 594
Formulation:
50 mM sodium borate with 0.05% sodium azide
Gene Symbols:
CDKN1B
Immunogen:
Purified GST-p27 fusion protein of human origin (Uniprot: P46527)
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
SX53G8
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Alias:
CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Host Species:
Mouse
Purification Method:
Protein A or G purified
Research Discipline:
Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Gene ID (Entrez):
1027.0
Target Species:
Human, Mouse, Rat, Primate
Isotype:
IgG1 κ

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Novus Biologicals

NP23471A32

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Antigen:
Bcl-xL
Classification:
Monoclonal
Conjugate:
Alexa Fluor 532
Formulation:
50 mM sodium borate with 0.05% sodium azide
Gene Symbols:
BCL2L1
Immunogen:
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (BX006 & 2H12) (Uniprot: Q07817)
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
BX006 + 2H12
Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Alias:
Apoptosis regulator Bcl-X, bcl2-L-1, BCL2-like 1, bcl-2-like protein 1, BCLXBCL2LBcl-X, bcl-xL, BCLXL, BCL-XL/S, bcl-xS, BCLXS, DKFZp781P2092
Host Species:
Mouse
Purification Method:
Protein A or G purified
Research Discipline:
Apoptosis, Biologically Active Proteins, Cancer, Phospho Specific, Tumor Suppressors
Gene ID (Entrez):
598.0
Target Species:
Human, Mouse, Rat, Porcine
Isotype:
IgG2a κ

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Novus Biologicals

NP234720A30

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Antigen:
TYRP1
Classification:
Monoclonal
Conjugate:
Alexa Fluor 350
Formulation:
50 mM sodium borate with 0.05% sodium azide
Gene Symbols:
TYRP1
Immunogen:
SK-MEL-23 cells
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Clone:
TA99
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen
Gene Alias:
b-PROTEIN, CAS25,6-dihydroxyindole-2-carboxylic acid oxidase, Catalase B, CATB, EC 1.14.18, EC 1.14.18.-, EC 1.14.18.1, Glycoprotein 75, GP75DHICA oxidase, Melanoma antigen gp75, TRP-1, TRPTYRP, tyrosinase-related protein 1TRP1, TYRRP
Host Species:
Mouse
Purification Method:
Protein A or G purified
Research Discipline:
Cancer
Gene ID (Entrez):
7306
Target Species:
Human, Mouse
Isotype:
IgG2a κ

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Novus Biologicals

NP234720A32

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Antigen:
TYRP1
Classification:
Monoclonal
Conjugate:
Alexa Fluor 532
Formulation:
50 mM sodium borate with 0.05% sodium azide
Gene Symbols:
TYRP1
Immunogen:
SK-MEL-23 cells
Quantity:
0.1 mL
Primary or Secondary:
Primary
Test Specificity:
It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Clone:
TA99
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen
Gene Alias:
b-PROTEIN, CAS25,6-dihydroxyindole-2-carboxylic acid oxidase, Catalase B, CATB, EC 1.14.18, EC 1.14.18.-, EC 1.14.18.1, Glycoprotein 75, GP75DHICA oxidase, Melanoma antigen gp75, TRP-1, TRPTYRP, tyrosinase-related protein 1TRP1, TYRRP
Host Species:
Mouse
Purification Method:
Protein A or G purified
Research Discipline:
Cancer
Gene ID (Entrez):
7306.0
Target Species:
Human, Mouse
Isotype:
IgG2a κ