NP247862A32

Nucleolin Antibody (364-5 + NCL/902), Alexa Fluor™ 532, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NP247862A32-Each-of-1 In Stock ₹ 55,271.76

NP247862A32 - Each of 1

₹ 55,271.76

In Stock

Quantity

1

Base Price: ₹ 55,271.76

GST (18%): ₹ 9,948.917

Total Price: ₹ 65,220.677

Antigen

Nucleolin

Classification

Monoclonal

Conjugate

Alexa Fluor 532

Formulation

50 mM sodium borate with 0.05% sodium azide

Gene Symbols

NCL

Immunogen

Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity

0.1 mL

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage

Store at 4°C in the dark.

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone

364-5 + NCL/902

Dilution

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species

Mouse

Purification Method

Protein A or G purified

Research Discipline

Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez)

4691.0

Target Species

Human, Bovine, Mouse, Rat

Isotype

IgG1 κ

Related Products

Img

Novus Biologicals

NP247860A30

--

Img

Novus Biologicals

NP247860A32

--

Img

Novus Biologicals

NP247860A94

--

Img

Novus Biologicals

NP23486A32

--

Img

Novus Biologicals

NP23486A94

--

Img

Novus Biologicals

NP23494A32

--

Img

Novus Biologicals

NP23486A30

--

Img

Novus Biologicals

NP23486A70

--

Description

  • Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Compare Similar Items

Show Difference

Img

Novus Biologicals

NP247862A32

--


Antigen:
Nucleolin

Classification:
Monoclonal

Conjugate:
Alexa Fluor 532

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4°C in the dark.

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
364-5 + NCL/902

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
4691.0

Target Species:
Human, Bovine, Mouse, Rat

Isotype:
IgG1 κ

Img

Novus Biologicals

NP247862A94

--


Antigen:
Nucleolin

Classification:
Monoclonal

Conjugate:
Alexa Fluor 594

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
NCL

Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Content And Storage:
Store at 4°C in the dark.

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
364-5 + NCL/902

Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence

Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Host Species:
Mouse

Purification Method:
__

Research Discipline:
__

Gene ID (Entrez):
4691

Target Species:
Human, Bovine, Mouse, Rat

Isotype:
IgG1 κ

Img

Novus Biologicals

NP247864A32

--


Antigen:
VCAM-1/CD106

Classification:
Monoclonal

Conjugate:
Alexa Fluor 532

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
VCAM1

Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This monoclonal antibody inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Content And Storage:
Store at 4°C in the dark.

Applications:
Flow Cytometry, Immunofluorescence

Clone:
B-K9

Dilution:
Flow Cytometry, Immunofluorescence

Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Host Species:
Mouse

Purification Method:
__

Research Discipline:
__

Gene ID (Entrez):
7412

Target Species:
Human, Rat

Isotype:
IgG1 κ

Img

Novus Biologicals

NP247864A70

--


Antigen:
VCAM-1/CD106

Classification:
Monoclonal

Conjugate:
Alexa Fluor 750

Formulation:
50 mM sodium borate with 0.05% sodium azide

Gene Symbols:
VCAM1

Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This monoclonal antibody inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Content And Storage:
Store at 4°C in the dark.

Applications:
Flow Cytometry, Immunofluorescence

Clone:
B-K9

Dilution:
Flow Cytometry, Immunofluorescence

Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

Host Species:
Mouse

Purification Method:
Protein A or G purified

Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Phospho Specific, Stem Cell Markers

Gene ID (Entrez):
7412.0

Target Species:
Human, Rat

Isotype:
IgG1 κ