MilliporeSigma™ anti-BRCA2 Clone: 2B,

Description

• Specifically detects BRCA2 Clone: 2B in Human samples, and it is validated for Immunoprecipitation Inherited mutations in the BRCA2 gene enhances the risk of familial breast cancer with the neoplasia related to loss of the wild type allele
• Human BRCA2 encodes a 3418 amino acid protein with little homology to any known proteins
• Transcription of BRCA2 appears to be co-ordinately regulated with BRCA1 during proliferation and differentiation with low level expression in early G1 and maximal expression occurring during the G1/S transition of the cell cycle
• While loss of BRCA2 is associated with early onset breast cancer, the gene is also critical for embryogenesis
• Although some mice nullizygous for BRCA2 die during embryogenesis, there have been BRCA2 knock-out mice that do survive to adulthood, albeit with various defects particularly in DNA repair capability
• Loss of BRCA2 expression in embryogenesis does not appear to affect apoptosis, per se, but proliferation is impaired, perhaps due to enhanced expression of p21WAF1
• Although the precise role for BRCA2 is still to be determined, recent studies implicate BRCA2 in DNA repair and recombination processes due to association with p53 and Rad51 and co-localization with BRCA1
• BRCA2 levels, as well as BRCA1 levels, decline following DNA damage and the decrease in BRCA2 accompanies activation of p53
• Interestingly, BRCA2 appears capable of specifically inhibiting p53 transcriptional activity, which implicates BRCA2 as a mediator of cell cycle control and DNA repair.