Eotaxin 3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-3 (CCL26)
• Anti-hEotaxin-3 (CCL26)-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin-3 (500-P156G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin-3
• Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P156GBT-1MG will be provided as 2 x 500 μg Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26
• Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors
• Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3
• It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4
• This gene for chemokine is located on human chromosome 7
• Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.