PIMA1202

Cas9 Monoclonal Antibody (10C11-A12), Invitrogen™

Manufacturer: Thermo Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PIMA1202-Each-of-1 In Stock ₹ 48,084.72

PIMA1202 - Each of 1

₹ 48,084.72

In Stock

Quantity

1

Base Price: ₹ 48,084.72

GST (18%): ₹ 8,655.25

Total Price: ₹ 56,739.97

Antigen

Cas9

Classification

Monoclonal

Concentration

1 mg/mL

Formulation

PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide

Gene Accession No.

Q99ZW2

Gene Symbols

cas9

Immunogen

Cas9 nuclease

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Bacteria

Product Type

Antibody

Isotype

IgG1 κ

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Clone

10C11-A12

Conjugate

Unconjugated

Gene

cas9

Gene Alias

cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus

Host Species

Mouse

Purification Method

Protein G

Regulatory Status

RUO

Gene ID (Entrez)

901176

Content And Storage

-20° C, Avoid Freeze/Thaw Cycles

Form

Liquid

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Description

  • Cas9 (10C11-A12) Mouse mAb recognizes transfected levels of total Cas9 protein
  • Stable Cas9 U2OS cell line used in validation experiments expresses Cas9 at lower levels
  • Assay results may vary depending on expression level of Cas9 in cell lines tested
  • This product was tested in Streptococcus pyogenes bacteria
  • Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering
  • The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence
  • It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome
  • In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation
  • Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9)
  • SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity
  • SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides
  • In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM
  • This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Compare Similar Items

Show Difference

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Thermo Scientific

PIMA1202

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Antigen:
Cas9

Classification:
Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide

Gene Accession No.:
Q99ZW2

Gene Symbols:
cas9

Immunogen:
Cas9 nuclease

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Bacteria

Product Type:
Antibody

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunocytochemistry, Western Blot

Clone:
10C11-A12

Conjugate:
Unconjugated

Gene:
cas9

Gene Alias:
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus

Host Species:
Mouse

Purification Method:
Protein G

Regulatory Status:
RUO

Gene ID (Entrez):
901176

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles

Form:
Liquid

Img

Thermo Scientific

PIMA120208

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Antigen:
VEGF Receptor 2

Classification:
Monoclonal

Concentration:
1.5 mg/mL

Formulation:
PBS with 1% BSA and 15mM sodium azide; pH 7.4

Gene Accession No.:
P35968

Gene Symbols:
KDR

Immunogen:
Recombinant, extracellular, human VEGF receptor 2 protein fragment.

Quantity:
50 μL

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1

Applications:
Flow Cytometry, Western Blot

Clone:
KDR-2 or 260.4

Conjugate:
Biotin

Gene:
KDR

Gene Alias:
6130401C07; CD309; CD309 antigen; fetal liver kinase 1; fetal liver kinase-1; FLK1; Flk-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2); FLK1 kinase insert domain receptor (VEGF receptor 2); flk-1 type VEGF receptor; KDR; kinase insert domain protein receptor; kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); kinase NYK; Krd-1; Ly73; protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; sVEGFR-2; tyrosine kinase growth factor receptor; vascular endotheial growth factor 2; vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR; VEGFR2; VEGFR-2

Host Species:
Mouse

Purification Method:
purified

Regulatory Status:
RUO

Gene ID (Entrez):
3791

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:
Liquid

Img

Thermo Scientific

PIMA120209

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Antigen:
CGRP

Classification:
Monoclonal

Concentration:
2.1 mg/mL

Formulation:
0.01M PBS with 15mM sodium azide; pH 7.4

Gene Accession No.:
P01256, P01257, P01258, P06881, P41547

Gene Symbols:
Calca

Immunogen:
Rat alpha-CGRP peptide.

Quantity:
50 μL

Primary or Secondary:
Primary

Target Species:
Canine, Human, Rat

Product Type:
Antibody

Isotype:
IgG1

Applications:
ELISA, Immunocytochemistry, Immunohistochemistry, Neutralization, Radioimmune Assays (RIA)

Clone:
4901

Conjugate:
Unconjugated

Gene:
Calca

Gene Alias:
alpha-type CGRP; CA; Cal1; CAL6; CALC; Calc1; Calca; calcitonin; calcitonin 1; Calcitonin carboxyl-terminal peptide; Calcitonin gene-related peptide 1; calcitonin gene-related peptide I; calcitonin related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; calcitonin; calcitonin gene-related peptide 1; calcitonin-related polypeptide alpha; CCALCI; CCP; Cgrp; CGRP1; CGRP-1; CGRP-I; Ct; Ctn; Katacalcin; KC; MGC126648; PCT; PDN-21; prepro-alpha-calcitonin gene-related peptide; RAT calcitonin; RATCAL6

Host Species:
Mouse

Purification Method:
purified

Regulatory Status:
RUO

Gene ID (Entrez):
24241, 403946, 796

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:
Liquid

Img

Thermo Scientific

PIMA120211

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Antigen:
SLC4A1

Classification:
Monoclonal

Concentration:
5.4 mg/mL

Formulation:
Ascites with 15mM sodium azide

Gene Accession No.:
P02730

Gene Symbols:
SLC4A1

Immunogen:
Purified, full length, native, human band 3 protein.

Quantity:
100 μL

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG2a

Applications:
ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot

Clone:
BIII 136

Conjugate:
Unconjugated

Gene:
SLC4A1

Gene Alias:
AE 1; AE1; anion exchange protein 1; anion exchanger 1; anion exchanger-1; band 3 anion transport protein; BND3; CAMP; CD233; CHC; DI; Diego blood group; EMPB3; EPB3; erythrocyte membrane protein band 3; erythroid anion exchange protein; FR; Froese blood group; l11Jus51; MEB3; MGC116750; MGC116753; MGC126619; MGC126623; RTA1A; SAO; Slc4a1; solute carrier family 4 (anion exchanger), member 1; solute carrier family 4 (anion exchanger), member 1 (Diego blood group); solute carrier family 4 member 1; solute carrier family 4 member 1 (Diego blood group); solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group); solute carrier family 4, anion exchanger, number 1; Solute carrier family 4, member 1, anion exchange protein 1 (kidney band 3); SPH4; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group; ZNF828

Host Species:
Mouse

Purification Method:
__

Regulatory Status:
RUO

Gene ID (Entrez):
6521

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form:
Liquid