LAMP2 Monoclonal Antibody (H4B4), Invitrogen™

Description

• MA1-205 Lamp2 antibody detects Lamp2 by western blot, immunofluorescence and flow cytometry
• MA1-205 Lamp2 antibody detects Lamp2 by western blot at ∽115kda which is larger than the protein itself due to heavy glycosylation
• LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins
• LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome
• Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide
• Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa
• LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail
• LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy
• LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail
• The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C
• LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane
• The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation
• LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).