CD34 Monoclonal Antibody (1H6), PE, Invitrogen™

Description

• Reconstitute with 1 mL of distilled water
• After reconstitution, store product undiluted at 4oC in the dark
• Once reconstituted, this product has a shelf life of approx
• 18 months
• For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
• Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa (McSweeney et al
• 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells
• CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations
• CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible
• CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors
• CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions
• CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells
• Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components
• CD34 is thought to have a role in presenting carbohydrate ligands to selectins
• The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction
• Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.