CD4/CD8 Antibody Cocktail, Invitrogen™

Description

• This CD4/CD8 monoclonal cocktail contains Mouse HRP CD4 (Clone 4B12, IgG1 kappa) and Rabbit AP CD8 (Clone SP16, IgG)
• It is designed for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissues using the MultiVision anti-mouse/HRP + anti-rabbit/AP detection system (Catalog No
• TL-012-MHRA)
• This cocktail is provided at an optimal concentration in an easy-to-use format for visualizing CD4 and CD8 lymphocyte subsets in red and blue, respectively
• The use of rabbit monoclonal CD8 allows for superior staining, in both sensitivity and specificity, permiting the use of higher titers compared to mouse monoclonal antibodies
• These cocktails are specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity
• The MultiVision AP and HRP polymers are innovative, patented technology
• The smaller amino acid polymer subunits minimize conflicts in binding the target protein resulting in more consistent staining and better signal amplification
• Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios
• This system is biotin-free, which prevents background staining found with traditional biotin-based methods
• The MultiVision polymer detection systems yield high quality double staining in less than 2 hours on formalin-fixed and paraffin-embedded tissue sections
• CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes
• It is also present on 80% of thymocytes and at a lower level on monocytes
• It is involved in recognition of antigen presented along with MHC class II by APCs
• It serves as receptor for HIV
• The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system
• The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules
• The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain
• Both alpha and beta chains share significant homology to immunoglobulin variable light chains.