P-Selectin Monoclonal Antibody (AK-6), Invitrogen™

Description

• For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
• A suggested positive control for immunohistochemical applications is tonsil
• This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using a sodium citrate buffer, pH6.0
• Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein
• P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling
• P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets
• From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate
• P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes
• P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes
• P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction
• Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.