DC-SIGN Monoclonal Antibody (MR-1), FITC, Invitrogen™

Description

• For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
• Mouse anti Human CD209 antibody, clone MR-1 recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a approximately 45 kDa C-type lectin that binds ICAM-3 also known as CD209
• CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro
• This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages
• The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health
• The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain
• The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells
• The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity
• Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability
• This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN)
• DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution
• Alternative splicing results in multiple variants.