E-selectin Recombinant Rabbit Monoclonal Antibody (105), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Cynomolgus E-Selectin/CD62e/SELE
• Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes
• In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation
• This response is immediate and involves a cascade of adhesion receptors
• At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade
• Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium
• E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines
• In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade
• E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.