PSME1 Recombinant Rabbit Monoclonal Antibody (016), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Human PA28A/PSME1
• Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors
• The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus
• Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum
• The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa
• It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes
• The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit
• An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation
• Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway
• Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.