Influenza A H1N1 M1 (A/Puerto Rico/8/34/Mount Sinai) Recombinant Rabbit Monoclonal Antibody (111), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for H1N1 (A/Puerto Rico/8/34/Mount Sinai) M1
• Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle
• M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription
• Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells
• Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts
• Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP
• During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP
• M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.