CD83 Recombinant Rabbit Monoclonal Antibody (005), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Mouse CD83
• CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells
• Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA
• In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres
• CD83 is also expressed by Langerhan's cells in the skin
• The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail
• CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein
• However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage
• CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages
• They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules
• Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.