5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody (GT513), Invitrogen™

Description

• Store as concentrated solution
• Centrifuge briefly prior to opening vial
• For short-term storage (1-2 weeks), store at 4°C
• For long-term storage, aliquot and store at -20°C or below
• Avoid multiple freeze-thaw cycles
• 5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA
• 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases
• Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells)
• A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine
• Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC
• This may open up entirely new perspectives in epigenetic studies.