ASIC1 Monoclonal Antibody (S271-44), PE, Invitrogen™

Description

• 1 μg/mL of MA5-45595 was sufficient for detection of ASIC1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.∣Detects approximately 60kDa
• This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily
• The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing
• The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown
• Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride
• ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum
• It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus
• Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.