CD20 Recombinant Rabbit Monoclonal Antibody (001), PE, Invitrogen™

Description

• Preparation: This antibody was obtained from a rabbit immunized with purified, Human CD20 Transfected CHO Cells and conjugated with PE under optimum conditions, the unreacted PE was removed
• Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity
• Protected from prolonged exposure to light
• Do not freeze! Sodium azide is toxic to cells and should be disposed of properly
• Flush with large volumes of water during disposal
• CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation
• CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells
• CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation
• CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation
• After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface
• CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment
• Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.