CD8a Recombinant Rabbit Monoclonal Antibody (208), PerCP, Invitrogen™

Description

• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD8 (Met1-Tyr196) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed
• Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity
• Protected from prolonged exposure to light
• Do not freeze! Sodium azide is toxic to cells and should be disposed of properly
• Flush with large volumes of water during disposal
• Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation
• CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules
• CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I
• CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha
• Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation
• Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells
• Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.