CD34 Recombinant Rabbit Monoclonal Antibody (013), APC, Invitrogen™

Description

• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD34 (Met1-Thr287) and conjugated with APC under optimum conditions, the unreacted APC was removed
• Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity
• Protected from prolonged exposure to light
• Do not freeze! CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations
• CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible
• CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors
• CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions
• CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells
• Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components
• CD34 is thought to have a role in presenting carbohydrate ligands to selectins
• The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction
• Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.