PIMA547317
Cas9 Monoclonal Antibody (3F9), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA547317-Each-of-1 | In Stock | ₹ 1,43,740.80 |
PIMA547317 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,43,740.80
GST (18%): ₹ 25,873.344
Total Price: ₹ 1,69,614.144
Antigen
Cas9
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 50% glycerol and 5mM sodium azide
Host Species
Mouse
Purification Method
Protein A/G
Regulatory Status
RUO
Target Species
Bacteria
Product Type
Antibody
Isotype
IgG1
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Clone
3F9
Conjugate
Unconjugated
Gene Alias
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
Immunogen
N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
Quantity
500 μL
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Description
- Western blot dilution: 1:1,000-1:2,000 (CAS9 transfected cells); 1:10,000-20,000 (pure full length CAS9 protein) Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering
- The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence
- It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome
- In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation
- Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9)
- SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity
- SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides
- In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM
- This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
Compare Similar Items
Show Difference
Antigen: Cas9
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: PBS with 50% glycerol and 5mM sodium azide
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Bacteria
Product Type: Antibody
Isotype: IgG1
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Clone: 3F9
Conjugate: Unconjugated
Gene Alias: cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
Immunogen: N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
Quantity: 500 μL
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Liquid
Antigen:
Cas9
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
PBS with 50% glycerol and 5mM sodium azide
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Bacteria
Product Type:
Antibody
Isotype:
IgG1
Applications:
Immunocytochemistry, Immunohistochemistry, Western Blot
Clone:
3F9
Conjugate:
Unconjugated
Gene Alias:
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
Immunogen:
N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
Quantity:
500 μL
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Liquid
Antigen: TDP-43
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: PBS with 50% glycerol and 5mM sodium azide
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Bovine, Equine, Human, Mouse, Porcine, Rat
Product Type: Antibody
Isotype: IgG1
Applications: Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot
Clone: 3H8
Conjugate: Unconjugated
Gene Alias: 1190002A23Rik; ALS10; C85084; hypothetical protein; OTTHUMP00000002171; OTTHUMP00000002173; RCJMB04_5g9; RP4-635E18.2; TAR DNA binding protein; TAR DNA binding protein L homeolog; TAR DNA-binding protein 43; TAR DNA-binding protein-43; tardbp; tardbp.L; Tdp43; TDP-43; wu:fb77f02; wu:fc52g10; XELAEV_18035188mg
Immunogen: Full length recombinant human TDP43 expressed in and purified from E. coli.
Quantity: 500 μL
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Liquid
Antigen:
TDP-43
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
PBS with 50% glycerol and 5mM sodium azide
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Bovine, Equine, Human, Mouse, Porcine, Rat
Product Type:
Antibody
Isotype:
IgG1
Applications:
Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot
Clone:
3H8
Conjugate:
Unconjugated
Gene Alias:
1190002A23Rik; ALS10; C85084; hypothetical protein; OTTHUMP00000002171; OTTHUMP00000002173; RCJMB04_5g9; RP4-635E18.2; TAR DNA binding protein; TAR DNA binding protein L homeolog; TAR DNA-binding protein 43; TAR DNA-binding protein-43; tardbp; tardbp.L; Tdp43; TDP-43; wu:fb77f02; wu:fc52g10; XELAEV_18035188mg
Immunogen:
Full length recombinant human TDP43 expressed in and purified from E. coli.
Quantity:
500 μL
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Liquid
Antigen: Pma1p
Classification: Monoclonal
Concentration: Conc. Not Determined
Formulation: antibody with 5mM sodium azide
Host Species: Mouse
Purification Method: __
Regulatory Status: RUO
Target Species: Bacteria, Yeast
Product Type: Antibody
Isotype: IgG
Applications: Immunocytochemistry, Western Blot
Clone: 40B7
Conjugate: Unconjugated
Gene Alias: PMA1
Immunogen: Yeast nuclear prep
Quantity: 2500 μL
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Liquid
Antigen:
Pma1p
Classification:
Monoclonal
Concentration:
Conc. Not Determined
Formulation:
antibody with 5mM sodium azide
Host Species:
Mouse
Purification Method:
__
Regulatory Status:
RUO
Target Species:
Bacteria, Yeast
Product Type:
Antibody
Isotype:
IgG
Applications:
Immunocytochemistry, Western Blot
Clone:
40B7
Conjugate:
Unconjugated
Gene Alias:
PMA1
Immunogen:
Yeast nuclear prep
Quantity:
2500 μL
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Liquid
Antigen: ALDH1L1
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: PBS with 50% glycerol and 5mM sodium azide
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Product Type: Antibody
Isotype: IgG2b
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Clone: 4A12
Conjugate: Unconjugated
Gene Alias: 10-formyltetrahydrofolate dehydrogenase; 10fTHF; 10-fTHF; 10FTHFDH; 10-FTHFDH; 1810048F20Rik; aldehyde dehydrogenase 1 family member L1; aldehyde dehydrogenase 1 family, member L1; aldehyde dehydrogenase family 1 member L1; Aldh1l1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; FBP-CI; FDH; formyltetrahydrofolate dehydrogenase; Fthfd; LOC100855730; MER57B-ALDH1L1; Neut2
Immunogen: Amino acids 1-401 of human ALDH1L1 expressed in and purified from E. coli.
Quantity: 500 μL
Primary or Secondary: Primary
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Liquid
Antigen:
ALDH1L1
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
PBS with 50% glycerol and 5mM sodium azide
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Product Type:
Antibody
Isotype:
IgG2b
Applications:
Immunocytochemistry, Immunohistochemistry, Western Blot
Clone:
4A12
Conjugate:
Unconjugated
Gene Alias:
10-formyltetrahydrofolate dehydrogenase; 10fTHF; 10-fTHF; 10FTHFDH; 10-FTHFDH; 1810048F20Rik; aldehyde dehydrogenase 1 family member L1; aldehyde dehydrogenase 1 family, member L1; aldehyde dehydrogenase family 1 member L1; Aldh1l1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; FBP-CI; FDH; formyltetrahydrofolate dehydrogenase; Fthfd; LOC100855730; MER57B-ALDH1L1; Neut2
Immunogen:
Amino acids 1-401 of human ALDH1L1 expressed in and purified from E. coli.
Quantity:
500 μL
Primary or Secondary:
Primary
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Liquid