Hepatitis B Virus Core/Ayw/Adw Monoclonal Antibody (1822), Invitrogen™

Description

• Specificity: Reactive with adw, ayw, and Hb core antigens
• Negative to normal human serum
• Best ELISA pairs (capture/conjugate): Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47486)/Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47487); Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47486)/Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47486); Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47486)/Hepatitis B Virus Core/Ayw/Adw Monoclonal antibody (Product # MA5-47488)
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• The hepatitis B virus is an important cause of acute and chronic liver disease
• It is also the most common form of parenterally transmitted viral hepatitis
• Hepatitis B virus is spherical in shape with a diameter of 42nm
• It contains a 27 nm partially double stranded DNA core enclosed within a lipoprotein coat
• The antigenic activity of the nucleocapsid core is designated as hepatitis B core antigen
• The antigens in the outer surface are called as hepatitis B virus surface antigens
• Core antigens are localized within the nuclei whereas the surface antigens are present in the cytoplasm of the infected cells
• Antibodies to surface antigens appear in circulation at an early stage of infection whereas the antibodies to the core antigens are detected after several weeks
• The virus is estimated to affect some 350 million people worldwide
• Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries
• Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus
• In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas
• There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.