SARS-CoV/SARS-CoV-2 NP Monoclonal Antibody (3862), Invitrogen™

Description

• Specificity: Specific for the nucleoprotein of the SARS virus (IFA & ELISA) and COVID-19 virus (ELISA)
• Negative for human coronaviruses 229E & OC43
• Reactive with the nucleocapsids of Variants: UK B.1.1.7, SA B.1.351, Brazil P.1 & B.1.617.2
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold
• Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb)
• The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells
• The spike protein is the main surface antigen of the coronavirus
• The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein
• This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics
• SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid
• It is the most abundant protein for coronavirus
• Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus.