Coronavirus Nucleoprotein Monoclonal Antibody (3877), Invitrogen™

Description

• Specificity: Reactive with the NP of native SARS, MERS by IFA & COVID-19
• Non-reactive with the following Coronavirus strains: 229E, NL63 & OC43
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• The coronavirus nucleocapsid (N) is a structural protein that forms complexes with genomic RNA, interacts with the viral membrane protein during virion assembly and plays a critical role in enhancing the efficiency of virus transcription and assembly
• Amino acid sequence comparisons have shown that coronavirus nucleocapsid proteins have three distinct and highly conserved domains: two structural and independently folded structural regions, namely the N terminal domain (NTD/domain 1) and C-terminal domain (CTD/domain 3), which are separated by a intrinsically disordered central region (RNA-binding domain/domain 2); all three domains have been shown in different CoVs to bind with viral RNA
• [PMID: 25105276]