Clostridium difficile GDH Monoclonal Antibody (5195), Invitrogen™

Description

• Specificity: Specific for glutamate dehydrogenase of C
• difficile
• Non-reactive with mammalian cells
• Best ELISA pairs (capture/conjugate): Clostridium difficile GDH Monoclonal antibody (Product # MA5-47560)/Clostridium difficile GDH Monoclonal antibody (Product # MA5-47561)
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• Clostridium difficile produces an NAD-specific glutamate dehydrogenase (GDH), which converts l-glutamate into alpha-ketoglutarate through an irreversible reaction
• The enzyme GDH is detected in the stool samples of patients with C
• difficile-associated disease and serves as one of the diagnostic tools to detect C
• difficile infection (CDI)
• [PMID: 24145018]