Shiga toxin 2B Monoclonal Antibody (7587), Invitrogen™

Description

• Specificity: Specific for Shiga toxin type II - B subunit (Stx-2b)
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• Shiga-like toxin (verotoxin) is a toxin produced by some strains of Escherichia coli
• Shiga-like toxin is named for its similarity to the AB5-type Shiga toxin produced by the bacteria Shigella dysenteriae
• There are two known types-SLT1 and SLT2
• The Shiga-like toxin is linked with hemolytic-uremic syndrome
• Shiga-like toxin requires highly specific receptors on the cells' surface in order to attach and enter the cell
• Species such as cattle, swine, and deer which do not carry these receptors may harbor toxigenic bacteria without any ill effect, dropping them in their feces, from where they may be distributed to humans
• Shiga Like Toxin-2 Subunit B has nontoxic action, it is the functional region which binds to the receptor
• Upon binding to the host cell receptor, the Shiga Like Toxin-2 Subunit B forms a pentamer
• The Shiga Like Toxin-2 Subunit B can be useful in vaccine study, antibody test and other functional research.