RSV Fusion Protein Monoclonal Antibody (0635), Invitrogen™

Description

• Specificity: specific for the fusion protein of RSV, types A&B
• Non-reactive with: Influenza A & B, Adenovirus, Para 1-3, Measles, Mumps
• Best ELISA pairs (capture/conjugate): RSV Fusion Protein Polyclonal antibody (Product # MA1-7286)/RSV Fusion Protein Monoclonal antibody (Product # MA5-47608); RSV Fusion Protein Monoclonal antibody (Product # MA1-7287)/RSV Fusion Protein Monoclonal antibody (Product # MA5-47608); RSV Fusion Protein Polyclonal antibody (Product # MA1-7081)/RSV Fusion Protein Monoclonal antibody (Product # MA5-47608); RSV Fusion Protein Monoclonal antibody (Product # MA5-47608)/RSV Fusion Protein Monoclonal antibody (Product # MA1-7287)
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• Human respiratory syncytial virus (RSV) is a member of the Paramyxoviridae family, subfamily Pneumovirinae, that causes common respiratory tract infections such as measles and mumps
• Its genome consists of single stranded, negative sense RNA that encodes three envelope glycoproteins, a small hydrophobic (SH) protein of unknown function, a major glycoprotein (G) known as the attachment protein, and a fusion (F) protein
• The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, resulting in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells
• Its name comes from the fact that F proteins on the surface of the virus cause the membranes on nearby cells to merge, forming syncytia
• While RSV typically produces mild symptoms in adults, it is one of the leading causes of lower respiratory tract infections and hospital visits during infancy and childhood.