CD40 Chimeric Recombinant Rabbit Monoclonal Antibody (5D12), Invitrogen™

Description

• Specificity: ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys
• The antibody requires residues within D1 and D1/B2 for binding (D1 domain is crucial for CD40L binding)
• Preparation: Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells
• The variable regions from regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12
• The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30
• CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells
• CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation
• AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions
• Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction
• The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis
• Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported
• Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.