NGF Polyclonal Antibody, Invitrogen™

Description

• Tested in vitro, this antibody abolishes the neuronal survival and the outgrowth actions of murine NGF in chicken DRG
• Reconstitute in 100 μl sterile water
• Centrifuge to remove insoluble material
• Use at a concentration of 1-5 μg/mL
• Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells
• The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites
• Although both types of receptor bind NGF only the high affinity form is capable of signal transduction
• Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex
• Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species
• NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons
• Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons
• Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.