beta-1 Adrenergic Receptor Polyclonal Antibody, Invitrogen™

Description

• PA1-049 detects beta-1 adrenergic receptor (B1AR) from , human, mouse, non-human primate, and rat tissues
• PA1-049 has been successfully used in Western blot and immunofluorescence procedures
• By Western blot, this antibody detects an ∽50.5 kDa band representing B1AR in mouse kidney membrane preparation
• Immunofluorescence staining of B1AR in mouse kidney distal tubule with PA1-049 yields a pattern consistent with plasma membrane staining
• The PA1-049 immunogen is a synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR
• This sequence is completely conserved between mouse and rat B1AR
• Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine
• Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family
• Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3)
• ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence
• BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism
• In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function
• Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system
• Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.