PPAR gamma-2 Polyclonal Antibody, Alexa Fluor™ 647, Invitrogen™

Description

• PA1-824 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human and mouse tissues
• This antibody does not detect PPAR alpha or PPAR delta
• PA1-824 immunogen is a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2
• This sequence is not conserved in PPAR gamma-1
• Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs)
• Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand
• Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids
• It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements
• PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids
• Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels
• PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids
• Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis
• PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.