PIPA5117622
E. coli (strain K12) H-NS Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5117622-Each-of-1 | In Stock | ₹ 45,560.70 |
PIPA5117622 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,560.70
GST (18%): ₹ 8,200.926
Total Price: ₹ 53,761.626
Antigen
E. coli (strain K12) H-NS
Classification
Polyclonal
Conjugate
Unconjugated
Gene
hns
Gene Alias
DNA-binding protein H-NS, Heat-stable nucleoid-structuring protein
Host Species
Rabbit
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
58460405, 75203350
Content And Storage
-20°C or -80°C if preferred
Form
Liquid
Applications
Western Blot
Concentration
2.28 mg/mL
Formulation
10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.
P0ACF8
Gene Symbols
hns
Immunogen
Recombinant Escherichia coli DNA-binding protein H-NS protein (1-137aa)
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Bacteria
Product Type
Antibody
Isotype
IgG
Related Products
Description
- This antibody has been tested in direct-ELISA A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed:333393, PubMed:2128918, PubMed:8890170, PubMed:8913298, PubMed:9398522, PubMed:16963779, PubMed:17046956, PubMed:23543115)
- Also involved in bacterial chromosome organization and compaction (PubMed:6379600, PubMed:10982869, PubMed:21903814)
- H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed:2512122, PubMed:16963779, PubMed:17435766, PubMed:17881364, PubMed:23543115)
- Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed:11714691)
- Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779, PubMed:17046956, PubMed:23543115)
- Many of these loci were horizontally transferred (HTG); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed:17046956, PubMed:17881364, PubMed:26789284)
- Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed:24449106)
- Repression of HTG by H-NS is thought to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional regulatory networks (PubMed:26789284)
- A subset of H-NSStpA-regulated genes also require Hha (andor Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NSStpA and may also modulate the oligomerization of the H-NSStpA-complex (PubMed:23543115)
- The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed:21903814)
- Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed:17435766, PubMed:17881364)
- Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed:8755860)
- May effect transcriptional elongation (PubMed:25638302)
- Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed:20595230)
- Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed:17010156)
- Plays a role in flagellar function (PubMed:11031114)
- Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed:20132443, PubMed:20659289)
- Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed:7934818)
- Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed:1537791)
- Has also been reported to activate transcription of some genes (PubMed:4566454, PubMed:338303, PubMed:2128918).
Compare Similar Items
Show Difference
Antigen: E. coli (strain K12) H-NS
Classification: Polyclonal
Conjugate: Unconjugated
Gene: hns
Gene Alias: DNA-binding protein H-NS, Heat-stable nucleoid-structuring protein
Host Species: Rabbit
Purification Method: Protein G
Regulatory Status: RUO
Gene ID (Entrez): 58460405, 75203350
Content And Storage: -20°C or -80°C if preferred
Form: Liquid
Applications: Western Blot
Concentration: 2.28 mg/mL
Formulation: 10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.: P0ACF8
Gene Symbols: hns
Immunogen: Recombinant Escherichia coli DNA-binding protein H-NS protein (1-137aa)
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Bacteria
Product Type: Antibody
Isotype: IgG
Antigen:
E. coli (strain K12) H-NS
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
hns
Gene Alias:
DNA-binding protein H-NS, Heat-stable nucleoid-structuring protein
Host Species:
Rabbit
Purification Method:
Protein G
Regulatory Status:
RUO
Gene ID (Entrez):
58460405, 75203350
Content And Storage:
-20°C or -80°C if preferred
Form:
Liquid
Applications:
Western Blot
Concentration:
2.28 mg/mL
Formulation:
10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.:
P0ACF8
Gene Symbols:
hns
Immunogen:
Recombinant Escherichia coli DNA-binding protein H-NS protein (1-137aa)
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Bacteria
Product Type:
Antibody
Isotype:
IgG
Antigen: Clostridium pasteurianum Rubredoxin
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Gene Alias: Rubredoxin (Rd)
Host Species: Rabbit
Purification Method: Protein G
Regulatory Status: RUO
Gene ID (Entrez): __
Content And Storage: -20°C or -80°C if preferred
Form: Liquid
Applications: Western Blot
Concentration: 3.5 mg/mL
Formulation: 10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.: __
Gene Symbols: __
Immunogen: Recombinant Clostridium pasteurianum Rubredoxin protein (1-54aa)
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Bacteria
Product Type: Antibody
Isotype: IgG
Antigen:
Clostridium pasteurianum Rubredoxin
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Gene Alias:
Rubredoxin (Rd)
Host Species:
Rabbit
Purification Method:
Protein G
Regulatory Status:
RUO
Gene ID (Entrez):
__
Content And Storage:
-20°C or -80°C if preferred
Form:
Liquid
Applications:
Western Blot
Concentration:
3.5 mg/mL
Formulation:
10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Recombinant Clostridium pasteurianum Rubredoxin protein (1-54aa)
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Bacteria
Product Type:
Antibody
Isotype:
IgG
Antigen: Ustilago sphaerogena RNU2
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Gene Alias: Ribonuclease U2; RNase U2
Host Species: Rabbit
Purification Method: Protein G
Regulatory Status: RUO
Gene ID (Entrez): __
Content And Storage: -20°C or -80°C if preferred
Form: Liquid
Applications: Western Blot
Concentration: 3.25 mg/mL
Formulation: 10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.: __
Gene Symbols: __
Immunogen: Recombinant Ustilago sphaerogena Ribonuclease U2 protein (1-114aa)
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Fungi
Product Type: Antibody
Isotype: IgG
Antigen:
Ustilago sphaerogena RNU2
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Gene Alias:
Ribonuclease U2; RNase U2
Host Species:
Rabbit
Purification Method:
Protein G
Regulatory Status:
RUO
Gene ID (Entrez):
__
Content And Storage:
-20°C or -80°C if preferred
Form:
Liquid
Applications:
Western Blot
Concentration:
3.25 mg/mL
Formulation:
10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Recombinant Ustilago sphaerogena Ribonuclease U2 protein (1-114aa)
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Fungi
Product Type:
Antibody
Isotype:
IgG
Antigen: Anas platyrhynchos Lysozyme C-1
Classification: Polyclonal
Conjugate: Unconjugated
Gene: __
Gene Alias: 1,4-beta-N-acetylmuramidase C; Lysozyme C type P; Lzp-s
Host Species: Rabbit
Purification Method: Protein G
Regulatory Status: RUO
Gene ID (Entrez): __
Content And Storage: -20°C or -80°C if preferred
Form: Liquid
Applications: Western Blot
Concentration: 2 mg/mL
Formulation: 10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.: __
Gene Symbols: __
Immunogen: Recombinant Anas platyrhynchos Lysozyme C-1 protein (19-147aa)
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Avian
Product Type: Antibody
Isotype: IgG
Antigen:
Anas platyrhynchos Lysozyme C-1
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
__
Gene Alias:
1,4-beta-N-acetylmuramidase C; Lysozyme C type P; Lzp-s
Host Species:
Rabbit
Purification Method:
Protein G
Regulatory Status:
RUO
Gene ID (Entrez):
__
Content And Storage:
-20°C or -80°C if preferred
Form:
Liquid
Applications:
Western Blot
Concentration:
2 mg/mL
Formulation:
10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
Recombinant Anas platyrhynchos Lysozyme C-1 protein (19-147aa)
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Avian
Product Type:
Antibody
Isotype:
IgG