PTPN2 Polyclonal Antibody, Invitrogen™

Description

• Positive test controls include: SW480, BT-474, Jurkat, K-562
• The target is usually found in the following locations: Cell membrane, Cytoplasm, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Nucleus
• Immunogen sequence: MPTTIEREFE ELDTQRRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK LQNAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRIVEKES VKCAQYWPTD DQEMLFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGD DINIKQVLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKCIK GDSSIQKRWK ELSKEDLSPA FDHSPNKIMT EKYNGNRIGL EEEKLTGDRC TGLSSKMQDT MEENSERPRL TDT T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins
• This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations
• The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors and cellular stress
• The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum 3 and seems to have distinctly different physiologic substrates from the smaller isoform
• Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types
• Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits 7, indicating a critical role for TC-PTP in bone marrow maturation
• TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52 Shc 6 and receptors for EGF 1, Insulin 8 and growth hormone.