SOX9b Polyclonal Antibody, Invitrogen™

Description

• Additional Information: No cross-reactivity with Sox9a
• This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:64,000
• Transcription factor that plays a key role in chondrocytes differentiation and skeletal development
• Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including COL2A1
• Plays a central role in successive steps of chondrocyte differentiation
• Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes
• Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis
• Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes
• Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells
• Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation
• In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells.