ATF6 Polyclonal Antibody, Invitrogen™

Description

• A suggested positive control is MDA-MB-361 cell lysate
• PA5-20216 can be used with blocking peptide PEP-0349
• ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes
• It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3')
• Binding to ERSE requires binding of NF-Y to ERSE
• ATF6 could also be involved in activation of transcription by the serum response factor
• ATF6 exists as a homodimer and heterodimer with ATF6 beta
• The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC)
• It Interacts also with the transcription factors GTF2I, YY1 and SRF
• Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus
• The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE
• During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis
• The cleavage seems to be performed sequentially by site-1 and site-2 proteases
• ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.