PD-1 Polyclonal Antibody, Invitrogen™

Description

• Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE
• A suggested positive control is THP-1 cell lysate
• PA5-20350 can be used with blocking peptide PEP-0470
• Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC)
• T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC
• PD-1 can bind two different but related ligands, PDL-1 and PDL-2
• Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of &quotedanger signals&quote such as LPS or other molecules associated with bacteria or other pathogens
• Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases
• Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.