LOK Polyclonal Antibody, Invitrogen™

Description

• Recommended positive controls: 293T, A431, HeLa, Raji
• Predicted reactivity: Mouse (94%), Rat (93%), Chicken (82%), Bovine (96%)
• Store product as a concentrated solution
• Centrifuge briefly prior to opening the vial
• Ubiquitin: exists either covalently attached to another protein, or free (unanchored)
• When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains)
• Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B
• Linear polymer chains formed via attachment by the initiator Met lead to cell signaling
• Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed
• When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling
• 60S ribosomal protein L40: component of the 60S subunit of the ribosome
• Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.