GPR149 Polyclonal Antibody, Invitrogen™

Description

• Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Elephant (94%); Monkey, Marmoset (88%); Bat (81%)
• For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer
• After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen
• This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member
• Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction
• Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation
• All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops
• A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits
• The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes.