STT3B Polyclonal Antibody, Invitrogen™

Description

• Immunogen sequence: NPPVEDSSDE DDKRNQGNLY DKAGKVRKHA TEQEKTEEGL GP Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 88%
• Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation
• N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER)
• All subunits are required for a maximal enzyme activity
• This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets
• STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A
• STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins
• Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation
• Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.