ZP3 Polyclonal Antibody, Invitrogen™

Description

• Immunogen sequence: QPLWLLQGGA SHPETSVQPV LVECQEATLM VMVSKDLFGT GKLIRAADLT LGPEACEPLV SMDTEDVVRF EVGLHECGNS MQVTD Highest antigen sequence identity to the following orthologs: Mouse - 60%, Rat - 58%
• The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo
• It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development
• The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction
• The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain
• It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix
• However, the requirement for furin cleavage in this process remains controversial based on mouse studies
• A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome
• Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.