MYO1A Polyclonal Antibody, Invitrogen™

Description

• Immunogen sequence: FSLHLSEMSSV GSKGDFLLVS EHVIELLTKM YRAVLDATQR QLTVTVTEKF SVRFKENSVA VKVVQGPAGG DNSKLRY Highest antigen sequence identity to the following orthologs - mouse 82%, rat 78%
• The protein encoded by this gene belongs to the myosin superfamily
• Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force
• Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences
• Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins
• At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location
• The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface
• Experiments indicate that the brush border population of the encoded protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively
• A rapidly exchanging pool of the protein encoded by this gene envelops an actin core bundle that, by comparison, is static in structure.