BAP1 Polyclonal Antibody, Invitrogen™

Description

• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1
• The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin
• In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals
• BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns
• BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth
• Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4
• 8-kb transcript expressed exclusively in testis
• Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling
• The BAP1 gene has been mapped to 3p21
• 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas
• Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines
• It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.