V5 Tag Monoclonal Antibody (SV5-Pk1), Invitrogen™

Description

• NOTE: R960-25 was previously sold under product #46-0705
• When re-ordering, please use #R960-25
• R960CUS was previously sold under product #46-1157
• When re-ordering, please use #R960CUS
• This antibody recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-
• This antibody is functionally tested against 20 ng of an E
• coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure
• This antibody has also been tested in Western blot against 25 ng of recombinant Positope™ protein
• The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5
• Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection
• For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM)
• Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates
• In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots
• This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope
• V5 Tag Monoclonal antibody specifically detects V5 Tag in Tag samples
• It is validated for ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot.