B6777

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

BioReagent, ≥99%, powder, suitable for molecular biology

Manufacturer: Sigma Aldrich

CAS Number: 6578-06-9

Synonym(S): 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt

Select a Size

Pack Size SKU Availability Price
100 MG B6777-100-MG In Stock ₹ 14,245.70
500 MG B6777-500-MG In Stock ₹ 42,737.10

B6777 - 100 MG

₹ 14,245.70

In Stock

Quantity

1

Base Price: ₹ 14,245.70

GST (18%): ₹ 2,564.226

Total Price: ₹ 16,809.926

product name

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BioReagent, for molecular biology, powder, ≥99%

grade

for molecular biology

Quality Level

200

product line

BioReagent

Assay

≥99%

form

powder

solubility

DMF: 20 mg/mLH2O: insoluble

foreign activity

Protease, none detected

storage temp.

−20°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

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Description

  • Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
  • Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
  • Substrates: Histochemical substrate for alkaline phosphatase.
  • Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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BioReagent

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