16670

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

≥95.0% (HPLC), powder

Manufacturer: Sigma Aldrich

CAS Number: 6578-06-9

Synonym(S): BCIP® p-toluidine salt, X-phosphate p-toluidine salt

Select a Size

Pack Size SKU Availability Price
100 MG 16670-100-MG In Stock ₹ 10,430.00
1 G 16670-1-G In Stock ₹ 57,640.00
5 G 16670-5-G In Stock ₹ 2,56,888.08

16670 - 100 MG

₹ 10,430.00

In Stock

Quantity

1

Base Price: ₹ 10,430.00

GST (18%): ₹ 1,877.40

Total Price: ₹ 12,307.40

product name

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥95.0% (HPLC)

Assay

≥95.0% (HPLC)

form

powder

storage temp.

2-8°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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Description

  • Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
  • Substrates: Histochemical substrate for alkaline phosphatase.
  • Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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16670

≥95.0% (HPLC), powder...


product name:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥95.0% (HPLC)

Assay:
≥95.0% (HPLC)

form:
powder

storage temp.:
2-8°C

SMILES string:
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI:
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key:
QEIFSLUFHRCVQL-UHFFFAOYSA-N

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Sigma Aldrich

1667122

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__

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Sigma Aldrich

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InChI:
1S/C7H7NOS/c9-7-5-2-4-10-6(5)1-3-8-7/h2,4H,1,3H2,(H,8,9)

InChI key:
SFNZVNVCJWVPDM-UHFFFAOYSA-N

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1S/C14H12ClNOS/c15-12-4-2-1-3-10(12)9-16-7-5-13-11(14(16)17)6-8-18-13/h1-4,6,8H,5,7,9H2

InChI key:
KQQBLQBNIVSMLU-UHFFFAOYSA-N